User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/09: Difference between revisions
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''Flourescent Microscopy''' | '''Flourescent Microscopy''' | ||
# Check RFP/CFP of [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/08 co-transfection] after 18 hrs. | # Check RFP/CFP of [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/08 co-transfection] after 18 hrs. | ||
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{| {{table}} cellspacing=''7'' | {| {{table}} cellspacing=''7'' | ||
|- valign="top" | |- valign="top" | ||
| '''BioBrick Part''' || '''Vector''' || '''Description''' || Microscopy Image | | '''BioBrick Part''' || '''Vector''' || '''Description''' || '''Microscopy Image''' || '''Comments""" | ||
|- | |- | ||
| KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS || [[Image:Merge-KAH59-PcVN.png | | | KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS || [[Image:Merge-KAH59-PcVN.png |250px]] || RFP and CFP signal | ||
|- | |- | ||
| KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS || [[Image:Merge-KAH60-PcVN.png | | | KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS || [[Image:Merge-KAH60-PcVN.png |250px]] || RFP and CFP signal | ||
|- | |- | ||
| KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS || [[Image:KAH54-PcVN.png | | | KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS || [[Image:KAH54-PcVN.png |250px]] || CFP signal only | ||
|- | |- | ||
| KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS || [[Image:Merged-KAH59(MV5).png | | | KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS || [[Image:Merged-KAH59(MV5).png |250px]] || RFP signal only | ||
|- | |- | ||
| KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS || [[Image:Merged-KAH66-MV5.png | | | KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS || [[Image:Merged-KAH66-MV5.png |250px]] || RFP and CFP signal | ||
|} | |} | ||
* Since RFP and CFP expressed at the same time, I concluded that both plasmids transfected to the U2OS cells and VP64 activated CFP expression. | |||
KAH54 has no mCherry sequence but VP64 could activate CFP expression and KAH59(MV5) without VP64 only shows RFP signal. |
Revision as of 17:29, 9 August 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
08/09/2013Flourescent Microscopy
KAH54 has no mCherry sequence but VP64 could activate CFP expression and KAH59(MV5) without VP64 only shows RFP signal. |