User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/08: Difference between revisions
From OpenWetWare
Line 58: | Line 58: | ||
Transgene expression should be detectable after 18 hours. | Transgene expression should be detectable after 18 hours. | ||
[http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/09 Flourescent microscopy] to check RFP/CFP expression. | [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/09 Flourescent microscopy] to check RFP/CFP expression. | ||
Revision as of 16:56, 9 August 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
08/08/2013 Gal4-VP64, Cyan Transactivation1 day before transfection:
Transfections > U2OS Flp-in T-REx lines (puro/zeo/blast) + FRT-marked mammalian transfection vector (hygro)
Transgene expression should be detectable after 18 hours. Flourescent microscopy to check RFP/CFP expression.
|