User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/06: Difference between revisions
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# Miniprep the transformed plasmids (GAL4-VP64) to test the transfection. | # Miniprep the transformed plasmids (GAL4-VP64) to test the transfection. | ||
# Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs. | # Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs. | ||
# Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo | # Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo | ||
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{| {{table}} | {| {{table}} | ||
|- | |- | ||
| Part number || 260 || | | Part number || 260/280 || ng/ul || 260/280 || ng/ul | ||
|- | |||
| KAH59 (MV5) || 140.198 || 1.857 || 277.719 || 1.832 | |||
|- | |||
| KAH54 (PCVN) || 169.122 || 1.824 || 217.267 || 1.832 | |||
|- | |- | ||
| | | KAH59 (PCVN) || 203.300 || 1.773 || 66.2 || 1.829 | ||
|- | |- | ||
| | | KAH60 (PCVN) ||33.877 || 1.988 || 169.931 || 1.71 | ||
|- | |- | ||
| | | KAH66 (MV5) || 121.409|| 1.814 || 174.819 || 1.822 | ||
|- | |||
| KAH36 || 162.434 || 1.832|| 130.043 || 1.832 | |||
|- | |- | ||
|} | |} | ||
* KAH59 (MV5) produce red pellet after transformed ''E-coli'' centrifugation. |
Revision as of 14:44, 8 August 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||
08/06/2013
Miniprep result
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