User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05

From OpenWetWare

< User:Behzad Damadzadeh | Notebook | PcTF Subcloning in E-coli | 2013 | 08(Difference between revisions)
Jump to: navigation, search
(Transformation)
Current revision (14:40, 7 August 2013) (view source)
(Date)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
=Date=
+
=08/06/2013=
=Transformation=
=Transformation=

Current revision

PcTF Subcloning in E. coli Main project page
Previous entry      Next entry

08/06/2013

Transformation

Transformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.

  • Parts
BioBrick Part Vector Description
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
KAH36 V0120 [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
  • 1μL of plasmid and 9μL dH2O.
  • 40μL of BL21-DE3 competent cells.
  • Culture on AMP agar plates in 37°C incubator overnight.
Personal tools