User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/08/05 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
 
Line 9: Line 9:
=Date=
=Date=


'''List title'''
=Transformation=
# List items
 
----
 
Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene.
 
'''Parts'''
 
{| {{table}} cellspacing=''7''
|- valign="top"
| '''BioBrick Part''' || '''Vector''' || '''Description'''
|-
| KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS
|-
| KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS
|-
| KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS
|-
| KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS
|-
| KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS
|-
| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector

Revision as of 16:11, 5 August 2013

PcTF Subcloning in E. coli <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Date

Transformation


Transformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.

Parts

BioBrick Part Vector Description
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
KAH36 V0120 [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector