User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05

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(Autocreate 2013/08/05 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
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'''List title'''
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=Transformation=
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# List items
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----
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Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene.
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'''Parts'''
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{| {{table}} cellspacing=''7''
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|- valign="top"
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| '''BioBrick Part''' || '''Vector''' || '''Description'''
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|-
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| KAH59 || PcVN || [K : Gal4 DB : mCh : mCh] + VP64-NLS
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|-
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| KAH60 || PcVN || [K : Gal4 DB : mCh] + VP64-NLS
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|-
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| KAH54 || PcVN || [[K : Gal4 DB] + VP64-NLS
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|-
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| KAH59 || PcN (MV5) || [K : Gal4 DB : mCh : mCh] + NLS
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|-
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| KAH66 || PcN (MV5) || [K : Gal4 DB : mCh : mCh : VP64] + NLS
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|-
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| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector

Revision as of 19:11, 5 August 2013

PcTF Subcloning in E. coli Main project page
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Date

Transformation


Transformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.

Parts

BioBrick Part Vector Description
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
KAH36 V0120 [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
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