User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05: Difference between revisions
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=Transformation= | =Transformation= | ||
Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene. | Transformation of Gal4-VP64 parts in '''BL21-DE3'''for transactivation of Cyan flourescent reporter gene. | ||
'''Parts''' | *'''Parts''' | ||
{| {{table}} cellspacing=''7'' | {| {{table}} cellspacing=''7'' | ||
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| KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector | | KAH36 || V0120 || [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector | ||
* 1μL of plasmid and 9μL dH2O. | |||
* 40μL of BL21-DE3 competent cells. | |||
* Culture on AMP agar plates in 37°C incubator overnight. |
Revision as of 16:14, 5 August 2013
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DateTransformationTransformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.
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