User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/17

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Transfer [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/10 Transfected Cells] from 6 well plate to 10cm cell culture dishes.
Transfer [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/10 Transfected Cells] from 6 well plate to 10cm cell culture dishes.
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Target cell concentrations are: 1*10
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*Add 2mL Trypsin to each of the wells 1 and 2 for 4 minutes.
*Add 2mL Trypsin to each of the wells 1 and 2 for 4 minutes.
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* Add 3.5mL media to neutralize Trypsin.

Revision as of 18:35, 23 July 2013

PcTF Subcloning in E. coli Main project page
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06/17/2013

Prepare Transfected Cells for Hygromycine Treatment

Transfer Transfected Cells from 6 well plate to 10cm cell culture dishes. Target cell concentrations are: 1*10


  • Add 2mL Trypsin to each of the wells 1 and 2 for 4 minutes.
  • Add 3.5mL media to neutralize Trypsin.
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