User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11

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(06/11/2013)
Current revision (18:30, 23 July 2013) (view source)
(06/11/2013)
 
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*BD002, No fluorescence was detected in cells  
*BD002, No fluorescence was detected in cells  
*Fluorescence protein is in low concentration dueweak  HSVtk TATA promoter,  
*Fluorescence protein is in low concentration dueweak  HSVtk TATA promoter,  
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*Next step: antibiotic colony selection technique
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*Next step: [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/21 antibiotic colony selection technique]
*Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection
*Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection

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06/11/2013

Transfection Fluorescent Microscopy

KAH187 BD002

  • Positive control,fluorescence was detected with Cyan filter.
  • BD002, No fluorescence was detected in cells
  • Fluorescence protein is in low concentration dueweak HSVtk TATA promoter,
  • Next step: antibiotic colony selection technique
  • Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection
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