User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11

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*Positive control,fluorescence was detected with Cyan filter.
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*BD002, No fluorescence was detected in cells
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*Fluorescence protein is in low concentration dueweak  HSVtk TATA promoter,
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*Next step: antibiotic colony selection technique
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*Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection

Revision as of 17:36, 23 July 2013

PcTF Subcloning in E. coli Main project page
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06/11/2013

Transfection Fluorescent Microscopy

KAH187 BD002

  • Positive control,fluorescence was detected with Cyan filter.
  • BD002, No fluorescence was detected in cells
  • Fluorescence protein is in low concentration dueweak HSVtk TATA promoter,
  • Next step: antibiotic colony selection technique
  • Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection
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