User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11: Difference between revisions
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*BD002, No fluorescence was detected in cells | *BD002, No fluorescence was detected in cells | ||
*Fluorescence protein is in low concentration dueweak HSVtk TATA promoter, | *Fluorescence protein is in low concentration dueweak HSVtk TATA promoter, | ||
*Next step: antibiotic colony selection technique | *Next step: [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/21 antibiotic colony selection technique] | ||
*Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection | *Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection | ||
Revision as of 16:30, 23 July 2013
 PcTF Subcloning in E. coli
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06/11/2013Transfection Fluorescent Microscopy
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