User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/06/11: Difference between revisions
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[ | *Positive control,fluorescence was detected with Cyan filter. | ||
*BD002, No fluorescence was detected in cells | |||
*Fluorescence protein is in low concentration dueweak HSVtk TATA promoter, | |||
*Next step: [http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/06/21 antibiotic colony selection technique] | |||
*Use Gal4-Vp64 transfection to screen for the positive colonies after the antibiotic selection |
Revision as of 16:30, 23 July 2013
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06/11/2013Transfection Fluorescent Microscopy
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