User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/04/22: Difference between revisions
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| Incubate at 37°C for 10 minutes. | | Incubate at 37°C for 10 minutes. | ||
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* '''Ligations''' | * '''Ligations''' | ||
{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u | | Ligation || <font color="blue"><u> | ||
|- | |- | ||
| 1. E. coli DH5α-T KAH182-KAH201(N/S)/size, 9.00 ng + V0200(N/S)/size, 48.00ng || <font color="blue">KAH182/KAH201 3:1 No Colonies</font> | | 1. E. coli DH5α-T KAH182-KAH201(N/S)/size, 9.00 ng + V0200(N/S)/size, 48.00ng || <font color="blue">KAH182/KAH201 3:1 No Colonies</font> | ||
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| 3. E. coli DH5α-T KAH182-KAH201(N/S)/size, 9.00 ng + V0200(N/S)/size, 48.00ng || <font color="blue">KAH182/KAH201 4:1 one Colony</font> | | 3. E. coli DH5α-T KAH182-KAH201(N/S)/size, 9.00 ng + V0200(N/S)/size, 48.00ng || <font color="blue">KAH182/KAH201 4:1 one Colony</font> | ||
|- | |- | ||
| 4. KAH201(S/P)/size, 36.29ng (Control Plate)|| <font color="blue"> | | 4. KAH201(S/P)/size, 36.29ng (Control Plate)|| <font color="blue">One Colonies and lots of satellite colonies in the middle of the pate</font> | ||
|} | |} |
Revision as of 13:19, 23 April 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DateBuilding a Reporter Gene to Determine Chromatin Protein Function (Ligation into the mammalian vector)
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