User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/04/22: Difference between revisions
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*Ligation of construct KAH182-KAH201 and mammalian vector V0200 and cloning in DH5α-T | *Ligation of construct KAH182-KAH201 and mammalian vector V0200 and cloning in DH5α-T. | ||
KAH182 = BBa_S04739 and KAH201 = BBa_S04745 and V0200 = BBa_J176121. | *KAH182 = BBa_S04739 and KAH201 = BBa_S04745 and V0200 = BBa_J176121. | ||
*Assembly strategy: The backbone V0200(N/S)/4442 , the insert is KAH182-KAH201(N/S)/1901. | *Assembly strategy: The backbone V0200(N/S)/4442 , the insert is KAH182-KAH201(N/S)/1901. | ||
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[[Image:KAH182-KAH201-V0200-S-N Digestion.jpg|350px| (1) KAH182-KAH201, 1901 bp, (2) V0200, 4442 bp, Both 1 and 2 are cut with Spe1 and Not11]] | [[Image:KAH182-KAH201-V0200-S-N Digestion.jpg|thumb|350px| (1) KAH182-KAH201, 1901 bp, (2) V0200, 4442 bp, Both 1 and 2 are cut with Spe1 and Not11]] | ||
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Revision as of 15:58, 22 April 2013
 PcTF Subcloning in E. coli
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DateBuilding a Reporter Gene to Determine Chromatin Protein Function (Ligation into the mammalian vector)
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