User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/04/18: Difference between revisions
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{| {{table}} cellspacing="3" width=350px | {| {{table}} cellspacing="3" width=350px | ||
| Plasmid DNA || | | Plasmid DNA || 25.00 μl | ||
|- | |- | ||
| Enzyme 1* || 1.0 μl | | Enzyme 1* || 1.0 μl | ||
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| Enzyme 2* || 1.0 μl | | Enzyme 2* || 1.0 μl | ||
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| 10x FastDigest buffer + green loading dye || | | 10x FastDigest buffer + green loading dye || 3.00 μl | ||
|- | |- | ||
| dH<sub>2</sub>O || 0.00 μl | | dH<sub>2</sub>O || 0.00 μl | ||
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'''Confirm The Assembly''' | '''Confirm The Assembly''' | ||
[[Image:KAH182- | [[Image:KAH201-KAH182-Ligation Confirmation.png|thumb|350px| Two seperate bands in each column show the vector backbone and the insert, 4400 bps and the KAH201+KAH182 (226+1675) constructed gene.Backbone, 1901 bps, Cut with EcoRI and PstI. First column: Ladder, Second collumn: 3:1 ratio, Third and fourth column: 4:1 ratio (colony A and B)]] | ||
{| | {| {{table}} cellspacing="3" width=400px | ||
| Plasmid DNA || 2.0 μl | | Plasmid DNA || 2.0 μl | ||
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| EcoR1 || 1.0 μl | | EcoR1 || 1.0 μl |
Revision as of 13:22, 6 May 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
04/18/2013Building a Reporter Gene to Determine Chromatin Protein Function
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