User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/01/24: Difference between revisions
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Ligation of NLS-HIS-STOP and V0120 vector in DH5α-T and BL21 | Ligation of NLS-HIS-STOP and V0120 vector in DH5α-T and BL21 | ||
* V0120 cut with X/P and purified from the gel. | * V0120 cut with X/P and purified from the gel. | ||
<div class="floatright">[[Image:Image:V0120(X-P).png ]]<br>V0120 Vector cut with Xba1 and Pst1 | |||
</div> | |||
* Ligations | * Ligations | ||
{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> |
Revision as of 12:12, 20 February 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/24/2013Making Standardized DNA Part (NLS-HIS-STOP) NLS-6His-STOP-F1 CTAGAcccaagaaaaagcgcaaggtacaccatcaccaccatcacgcgtaaagctgagACTAGTAGCGGCCGCTGCA 76 NLS-6His-STOP-R1 GCGGCCGCTACTAGTctcagctttacgcgtgatggtggtgatggtgtaccttgcgctttttcttgggT 68 Set up an annealing reaction as follows:
Heat at 100°C for 5 min., remove the entire heat block or water bath from the heat source, and allow to cool slowly to room temperature.
File:Image:V0120(X-P).png
V0120 Vector cut with Xba1 and Pst1
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