User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/01/12: Difference between revisions
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* Calculations are for the ng of insert we need to get a 3:1 ratio of insert molecules to 50 ng vector molecules | |||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
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* The incubation time for the ligation process was 30min at room temperature. | * The incubation time for the ligation process was 30min at room temperature. | ||
* Fast transformation, 30 min on ice. |
Revision as of 14:52, 12 January 2013
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/12/2013Ligation of KAH111, KAH112 and C-His vector in DH5α-T and BL21
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