Revision as of 17:09, 12 January 2013

PcTF Subcloning in E. coli

01/12/2013

Ligation of KAH111, KAH112 and C-His vector in DH5α-T and BL21

The 4 μL of C-His Vector from stock is diluted with 16 μL of nuclease free water, then cut with E/P and purified from the gel.
Ligations

Ligation	</font>
1. E. coli BL-21 KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng	KAH111/C-His 3:1 No Colonies
2. E. coli DH5α-T KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 26ng	KAH111/C-His 3:1 No Colonies
3. E. coli BL-21 KAH112(E/P)/size, 22.8 ng + C-His(E/P)/3600, 26ng	KAH112/C-His 3:1 No Colonies
4. E. coli DH5α-T KAH111(E/P)/size, 20 ng + C-His(E/P)/3600, 26ng	KAH112/C-His 3:1 No Colonies
5. C-His(E/P)/ 26 ng (Control Plate)

@@ Line 39: / Line 39: @@
 | dH<sub>2</sub>O    || 0 || 0 || 0.03  || 0.03 || 2.08
 |-
-| &nbsp;             || 10.52 μL || 10.52 μL || 10μl || 10μL
+| &nbsp;             || 10.52 μL || 10.52 μL || 10μl || 10μL || 10μL
 |}