User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/20

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'''1-Step Human Coupled in vitro protein expression'''
'''1-Step Human Coupled in vitro protein expression'''
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# List items
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* 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction.
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[http://www.piercenet.com/browse.cfm?fldID=0F8AB1C8-B7C2-3CC6-6B59-9BC58F0BA912 Kit Specifications]
[http://www.piercenet.com/browse.cfm?fldID=0F8AB1C8-B7C2-3CC6-6B59-9BC58F0BA912 Kit Specifications]
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* The PcTF gene with two different backbones were used for the protein expression.
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** pCFE1-CHis Expression vector
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** Mv2 vector , Part:BBa_J176122
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'''Table of Components for the IVT reaction'''

Revision as of 19:58, 20 December 2012

PcTF Subcloning in E. coli Main project page
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12/20/2012

1-Step Human Coupled in vitro protein expression

  • 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100µg/mL per reaction.

Kit Specifications

  • The PcTF gene with two different backbones were used for the protein expression.
    • pCFE1-CHis Expression vector
    • Mv2 vector , Part:BBa_J176122

Table of Components for the IVT reaction

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