User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/18

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(Autocreate 2012/12/18 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
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=18/12/2012=
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'''List title'''
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'''RFP Plate Reader Serial Dilution'''
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# List items
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# Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution)
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# Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution)
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# Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution)
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# Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution)
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'''Plate Reader'''
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* Transfer 50 μl of each solution to the Costar 96 clear bottom black side.
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* Here is the software setting:
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** Fluorescence
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** Endpoint
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** Full Plate
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** Excitation: 540,  Emission: 600
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** Optics: Top
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** Gain: AutoScale
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** Light Source: Xenon Flash,  Lamp Energy: High
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** Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
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** Read Height: 9.5 mm

Revision as of 17:02, 18 December 2012

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18/12/2012

RFP Plate Reader Serial Dilution

  1. Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution)
  2. Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution)
  3. Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution)
  4. Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution)

Plate Reader

  • Transfer 50 μl of each solution to the Costar 96 clear bottom black side.
  • Here is the software setting:
    • Fluorescence
    • Endpoint
    • Full Plate
    • Excitation: 540, Emission: 600
    • Optics: Top
    • Gain: AutoScale
    • Light Source: Xenon Flash, Lamp Energy: High
    • Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
    • Read Height: 9.5 mm
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