User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/18: Difference between revisions
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= | =18/12/2012= | ||
''' | '''RFP Plate Reader Serial Dilution''' | ||
# | # Transfer 100 μl of RFP from stock to 100μl DI water. (50% solution) | ||
# Transfer 100 μl of 50% solution to 100 μl DI water. (25% solution) | |||
# Transfer 100 μl of 25% solution to 100 μl DI water. (12.5% solution) | |||
# Transfer 100 μl of 12.5% solution to 100 μl DI water. (6.25% solution) | |||
'''Plate Reader''' | |||
* Transfer 50 μl of each solution and a control to the Costar 96 clear bottom black side. | |||
* Here is the software setting: | |||
** Fluorescence | |||
** Endpoint | |||
** Full Plate | |||
** Excitation: 540, Emission: 600 | |||
** Optics: Top | |||
** Gain: AutoScale | |||
** Light Source: Xenon Flash, Lamp Energy: High | |||
** Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10 | |||
** Read Height: 9.5 mm | |||
* Click OK and then Run the experiment. | |||
* Export the data to an excel file. | |||
* The software will deduce the control data from the sample data | |||
'''Result''' | |||
{| border="1" | |||
! Dilution !! RFP (AU) | |||
|- | |||
|format modifier (not displayed) | 100% ||43125 | |||
|- | |||
|format |50% ||format | 16143 | |||
|- | |||
|format |25% ||format | 7978 | |||
|- | |||
|format |12.5% ||format | 4124 | |||
|- | |||
|format |6.25% ||format | 2718 | |||
|} |
Revision as of 14:23, 18 December 2012
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18/12/2012RFP Plate Reader Serial Dilution
Plate Reader
Result
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