Revision as of 19:52, 14 December 2012

PcTF Subcloning in E. coli

14/12/2012

Ligation	Plate results (lig : neg crtl) 12/12/12
1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng	KAH111/C-His 3:1 (Pick 4)
2. E. coli BL-21 KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng	KAH111/C-His 3:1 (Pick 4)
3. C-His(E/P)/ 25 ng

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 <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-=Date=
+=14/12/2012=
-'''List title'''
+* Ligations
-# List items
+{| {{table}} cellspacing="3" <!-- Ligations table -->
+|- bgcolor=#cfcfcf
+| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/12/12</font>
+|-
+| 1. E. coli NEB10B KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 (Pick 4)</font>
+|-
+| 2. E. coli BL-21   KAH111(E/P)/size, 16 ng + C-His(E/P)/3600, 25ng || <font color="blue">KAH111/C-His 3:1 (Pick 4)</font>
+|-
+| 3. C-His(E/P)/ 25 ng ||
+|}
+{| {{table}} cellspacing="3" <!-- Ligation rxn table -->
+| &nbsp;             || 1    || 2  || 3
+|-
+| Insert DNA         || 3.12  || 3.12 || ---
+|-
+| Vector DNA         || 2.94  || 2.94 || 2.94
+|-
+| 2x lgn buf (Roche) || 7.06  || 7.06 || 5.0
+|-
+| T4 ligase (NEB)    || 1.0  || 1.0  || 1.0
+|-
+| dH<sub>2</sub>O    || 0 || 0 || 0.88
+|-
+| &nbsp;             || 14.12 μL || 14.12 μL || 10μl
+|}