Revision as of 22:41, 2 December 2012

PcTF Subcloning in E. coli

12/1/12

> Digests (Fermentas FD)
PT7CFE1-CHis

2 Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.

Plasmid DNA	3.0 μl*
EcoR1	1.0 μl
Pst1	1.0 μl
10x FastDigest buffer + green loading dye	1.5 μl
dH₂O	8.5 μl
	15.0 μl total
Incubate at 37°C for 10 minutes.

Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.

@@ Line 7: / Line 7: @@
 <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-=Date=
+=12/1/12=
-'''List title'''
+> Digests (Fermentas FD)<br>
-# List items
+PT7CFE1-CHis
+[[Image:Vector_Cut_(E-P).png‎ |thumb|350px|2 Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.]]
+{| class="wikitable" width=400px
+| Plasmid DNA || 3.0 μl*
+|-
+| EcoR1  || 1.0 μl
+|-
+| Pst1 || 1.0 μl
+|-
+| 10x FastDigest buffer + green loading dye || 1.5 μl
+|-
+| dH<sub>2</sub>O || 8.5 μl
+|-
+| &nbsp; || 15.0 μl total
+|-
+| Incubate at 37°C for 10 minutes.
+|}
+# Make the (1%) agarose glee and add 15μl  of restricted vector in one well and 10 μl  of ladder.