User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/12/01

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Current revision (22:02, 2 December 2012) (view source)
(12/1/12)
 
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> Digests (Fermentas FD)<br>
> Digests (Fermentas FD)<br>
 +
PT7CFE1-CHis
PT7CFE1-CHis
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[[Image:Vector_Cut_(E-P).png‎ |thumb|350px|2 Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.]]
+
[[Image:Vector_Cut_(E-P).png‎ |thumb|350px| Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.]]
{| class="wikitable" width=400px  
{| class="wikitable" width=400px  
| Plasmid DNA || 3.0 μl*
| Plasmid DNA || 3.0 μl*

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12/1/12

> Digests (Fermentas FD)

PT7CFE1-CHis

Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.
Human Vector PT7CFE1-CHis, 3600 bp, Cut with EcoRI and PstI Separately.
Plasmid DNA 3.0 μl*
EcoR1 1.0 μl
Pst1 1.0 μl
10x FastDigest buffer + green loading dye 1.5 μl
dH2O 8.5 μl
  15.0 μl total
Incubate at 37°C for 10 minutes.
  1. Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
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