User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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|- valign="top" | |- valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || | | <u>Reagent</u> || <u>Volume</u> || | ||
| rowspan="9" | <!--- [[Image: | | rowspan="9" | [[Image:PcTF_in-vitro_Expression.png |thumb|350px| 1. KAH109, 2. KAH111, 3. KAH112, 4. KAH115, 5. KAH148, 6. KAH150, 7. KAH151 8. Human Vector PT7CFE1-CHis(3600 bp) Cut with EcoRI and PstI]] | ||
<!--- [[Image:PcTF_in-vitro_Expression.png|350px|Cloning digest ]]<br>30 μL/lane, 1% agarose ---> | |||
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| DNA (plasmid) || 25.0 μL | | DNA (plasmid) || 25.0 μL | ||
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| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | ||
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| 1. pT7CFE1-CHis (E/P) || | | 1. pT7CFE1-CHis (E/P) || --- || 1.829 || 57.344 | ||
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| 2. KAH109, (E/P) || --- || | | 2. KAH109, (E/P) || --- || 2.354 || 36.072 | ||
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| 3. KAH111, (E/P) || --- || | | 3. KAH111, (E/P) || --- || 2.238 || 15.003 | ||
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| 4. KAH112, (E/P) || --- || | | 4. KAH112, (E/P) || --- || 1.862 || 22.857 | ||
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| 5. KAH115, (E/P) || --- || | | 5. KAH115, (E/P) || --- || 2.096 || 18.312 | ||
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| 6. KAH148, (E/P) || --- || | | 6. KAH148, (E/P) || --- || 2.389 || 23.735 | ||
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| 7. KAH150, (E/P) || --- || | | 7. KAH150, (E/P) || --- || 1.99 || 19.822 | ||
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| 8. KAH151, (E/P) || --- || | | 8. KAH151, (E/P) || --- || 1.90 || 19.701 | ||
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* Note: Use a 3:1 ratio of insert to vector... x μL insert = 3 * (bp of insert/ bp of vector) * 50 ng vector / (concentration of insert ng/μL) | * Note: Use a 3:1 ratio of insert to vector... x μL insert = 3 * (bp of insert/ bp of vector) * 50 ng vector / (concentration of insert ng/μL) | ||
# BD-116 ( | # BD-116 (KAH109) | ||
# | # BD-117 (KAH111) | ||
# | # BD-118 (KAH112) | ||
# | # BD-119 (KAH115) | ||
# | # BD-120 (KAH148) | ||
# | # BD-121 (KAH150) | ||
# | # BD-122 (KAH-151) | ||
# | # CTRL | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> |
Latest revision as of 22:16, 26 September 2017
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11/20/12Assemblies: Shown as Part/cuts/purified fragment size
Measure concentration(s)
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