User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
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# KAH150/pT7CFE1-CHis: KAH190/(E/S)/967 + " | # KAH150/pT7CFE1-CHis: KAH190/(E/S)/967 + " | ||
# KAH151/pT7CFE1-CHis: KAH191/(E/S)/967 + " | # KAH151/pT7CFE1-CHis: KAH191/(E/S)/967 + " | ||
Note: KAH109 is being re-done as an internal control to trouble-shoot the ligation<br> | |||
Note: KAH111-KAH151 are all cut and purified already | |||
> Digest (Fermentas FD)<br> | |||
# pT7CFE1-CHis, E/P | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:KAH_060311_gel1.tif|350px|Cloning digests 6/03/11]]<br>30 μL/lane, 1% agarose | |||
|- | |||
| DNA (plasmid) || 25.0 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| EcoRI || 1.0 | |||
|- | |||
| PstI || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 0 | |||
|- | |||
| || 30 μL --> 37°C/ ~30 min. | |||
|} | |||
Revision as of 11:54, 16 November 2012
 PcTF Subcloning in E. coli
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
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