User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PcTF Subcloning in E. coli</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 27: | Line 27: | ||
'''Digest (Fermentas FD)'''<br> | '''Digest (Fermentas FD)'''<br> | ||
# pT7CFE1-CHis, E/P | # pT7CFE1-CHis, E/P | ||
# KAH109 E/P | |||
# KAH111 E/P | |||
# KAH112 E/P | |||
# KAH115 E/P | |||
# KAH148 E/P | |||
# KAH150 E/P | |||
# KAH151 E/P | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || | | <u>Reagent</u> || <u>Volume</u> || | ||
| rowspan="9" | | | rowspan="9" | [[Image:PcTF_in-vitro_Expression.png|350px|Cloning digest ]]<br>30 μL/lane, 1% agarose | ||
|- | |- | ||
| DNA (plasmid) || 25.0 μL | | DNA (plasmid) || 25.0 μL | ||
Line 70: | Line 77: | ||
|} | |} | ||
'''Ligations''' (trouble shooting suggestions) | '''Ligations''' (trouble shooting suggestions) | ||
Line 101: | Line 100: | ||
|} | |} | ||
'''Plates Nomenclature | |||
# BD-116, (KAH109) | |||
# BD-117, (KAH111) | |||
# BD-118, (KAH112) | |||
# BD-119, (KAH115) | |||
# BD-120, (KAH148) | |||
# BD-121, (KAH150) | |||
# BD-122, (KAH151) | |||
# CTRL | |||
* Add total ligation reaction to 30 μL DH5α-Turbo cells | * Add total ligation reaction to 30 μL DH5α-Turbo cells | ||
* Follow routine "fast" transformation procedure | * Follow routine "fast" transformation procedure | ||
* Plate onto 100 ug/mL Amp agar plates | * Plate onto 100 ug/mL Amp agar plates |
Latest revision as of 22:16, 26 September 2017
PcTF Subcloning in E. coli | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
Measure concentration(s)
Plates Nomenclature
|