User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/11/16: Difference between revisions
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'''Digest (Fermentas FD)'''<br> | '''Digest (Fermentas FD)'''<br> | ||
# pT7CFE1-CHis, E/P | # pT7CFE1-CHis, E/P | ||
# KAH109 E/P | |||
# KAH111 E/P | |||
# KAH112 E/P | |||
# KAH115 E/P | |||
# KAH148 E/P | |||
# KAH150 E/P | |||
# KAH151 E/P | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || | | <u>Reagent</u> || <u>Volume</u> || | ||
| rowspan="9" | | | rowspan="9" | [[Image:PcTF_in-vitro_Expression.png|350px|Cloning digest ]]<br>30 μL/lane, 1% agarose | ||
|- | |- | ||
| DNA (plasmid) || 25.0 μL | | DNA (plasmid) || 25.0 μL | ||
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|} | |} | ||
'''Ligations''' (trouble shooting suggestions) | '''Ligations''' (trouble shooting suggestions) | ||
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|} | |} | ||
'''Plates Nomenclature | |||
# BD-116, (KAH109) | |||
# BD-117, (KAH111) | |||
# BD-118, (KAH112) | |||
# BD-119, (KAH115) | |||
# BD-120, (KAH148) | |||
# BD-121, (KAH150) | |||
# BD-122, (KAH151) | |||
# CTRL | |||
* Add total ligation reaction to 30 μL DH5α-Turbo cells | * Add total ligation reaction to 30 μL DH5α-Turbo cells | ||
* Follow routine "fast" transformation procedure | * Follow routine "fast" transformation procedure | ||
* Plate onto 100 ug/mL Amp agar plates | * Plate onto 100 ug/mL Amp agar plates |
Revision as of 13:46, 26 November 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/16/12
Assemblies: Shown as Part/cuts/purified fragment size
Measure concentration(s)
Plates Nomenclature
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