User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/05/22: Difference between revisions
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# Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down). | # Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down). | ||
# Grow the cultures overnight in a shaking 37°C incubator. | # Grow the cultures overnight in a shaking 37°C incubator. | ||
'''BugBuster Protein Extraction''' | |||
#Harvest cells from liquid culture by centrifugation at 16000 ×g for 10 minutes. | |||
# Weighed |
Revision as of 16:21, 24 May 2012
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5/22/2012PcTF Exctraction from E-coli Transform bacteria with the ligated plasmids 30 minutes
Note: The negative control will show you the number of “background” colonies so that you can determine whether your transformation worked, or is just the result of vector self-ligation or selection failure. Grow liquid cultures
BugBuster Protein Extraction
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