User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/11: Difference between revisions
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'' | > Digests (Fermentas FD)<br> | ||
BD-112 and BD-113 | |||
[[Image:BD-111-Behzad-5-4-12.jpg|thumb|350px|White dashed lines border show the vector backbone and the BD-111 constructed gene(RBS + PcTF).]] | |||
{| class="wikitable" width=400px | |||
| Plasmid DNA || 2.0 μl* | |||
|- | |||
| EcoR1 || 1.0 μl | |||
|- | |||
| Pst1 || 1.0 μl | |||
|- | |||
| 10x FastDigest buffer + green loading dye || 1.5 μl | |||
|- | |||
| dH<sub>2</sub>O || 9.5 μl | |||
|- | |||
| || 15.0 μl total | |||
|- | |||
| Incubate at 37°C for 10 minutes. | |||
|} | |||
# Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder. | |||
'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp' |
Revision as of 10:00, 11 April 2012
PcTF Subcloning in E. coli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||
11/4/12> Digests (Fermentas FD)
'The size of the constructed gene supposed to be: 1123 + 15 + 6 = 1144 bp' |