User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2012/04/05: Difference between revisions
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''' | '''Culturing the colonies grew on agar plates on 4/4/2012''' | ||
# | # BBa_I712074 | ||
# BBa_I719005 | |||
# BBa_B0030 | |||
# BD111 | |||
'''Grow liquid cultures''' | |||
# Label 15 ml sterile culture tube(s) appropriately. Fill each tube with 2 ml of LB growth medium + appropriate antibiotic (e.g., 100 μg/ml ampicillin). | |||
# Using a sterile pipette tip, touch the bacterial streak (or pick up a single colony) and put the tip into the LB medium (bacterial end down). | |||
# Grow the cultures for 7 hours in a shaking 37°C incubator.<br><br> |
Revision as of 11:40, 5 April 2012
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5/4/2012Culturing the colonies grew on agar plates on 4/4/2012
Grow liquid cultures
|