User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

04/04/11

• ✓ Assemblies: BD111, BD112, BD113

Assemblies

1. BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
2. BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
3. BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23

> Digests (Fermentas FD)

Reagent	Volume		30 μL/lane, 1% agarose -->
DNA (plasmid)	up to 20 μL
10x buffer	3.0
enzyme 1	1.0
enzyme 2	1.0
dH2O	---
	30 μL --> 37°C/ ~30 min.