User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

Project Description/Abstract

• Synthetic biology is an exciting new field that aims to create novel biological functions by artificially combining natural molecular parts. Rational design allows us to engineer the behaviors of protein-based drugs, in ways that we cannot accomplish with small molecule-based drugs. DNA assembly will be used to build a combinatorial library of over 200 synthetic transcription factors (Pc-Tfs) that will potentially activate therapeutic genes in cancer and stem cells. As time allows, a select group of Pc-TF protein will be purified and analyzed biochemically.

Notes

• Brady Laughlin's Spring 2012 project in the Haynes Lab at Arizona State University
• BME 394 Honors Research Project

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