User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

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BioBrick Part Part Number Plate Well Backbone Primer Length Fw Bw T7 promoter (strong promoter from T7 bacteriophage) BBa_I712074 2011 Kit Plate 1 6N pSB1AK8 46 bp T7 Promoter BBa_I719005 2011 Kit Plate 1 15N pSB1A2 VR VF2 23 bp RBS (Elowitz 1999) -- defines RBS efficiency BBa_B0034 2011 Kit Plate 1 2M pSB1A2 VR VF2 12bp RBS.1 (strong) -- modified from R. Weiss BBa_B0030 2011 Kit Plate 1 1H pSB1A2 VR VF3 15bp




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