User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

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==04/04/11==
 
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<!-- Precede finished items with a checkmark &#x2713; -->
 
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* &#x2713; Assemblies: BD111, BD112, BD113
 
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----
 
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'''Assemblies'''
 
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# BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
 
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# BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
 
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# BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23
 
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> Digests (Fermentas FD)<br>
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==Behzad's Notebook==
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
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| DNA (plasmid) || up to 20 μL
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| 10x buffer || 3.0
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| enzyme 1 || 1.0
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| enzyme 2 || 1.0
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| dH<sub>2</sub>O || ---
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| &nbsp; || 30 μL --> 37°C/ ~30 min.
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|}
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__NOTOC__
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'''Project Description'''
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* Project description coming soon

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