User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(One intermediate revision not shown.)
Line 13: Line 13:
|-  
|-  
|colspan="2"|
|colspan="2"|
-
==04/04/11==
 
-
<!-- Precede finished items with a checkmark &#x2713; -->
 
-
* &#x2713; Assemblies: BD111, BD112, BD113
 
-
----
 
-
'''Assemblies'''
 
-
# BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
 
-
# BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
 
-
# BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23
 
 +
==Behzad's Notebook==
-
> Digests (Fermentas FD)<br>
+
'''Project Description'''
-
 
+
* Project description coming soon
-
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
+
-
|-valign="top"
+
-
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
+
-
| rowspan="9" |
+
-
|-
+
-
| DNA (plasmid) || up to 20 μL
+
-
|-
+
-
| 10x buffer || 3.0
+
-
|-
+
-
| enzyme 1 || 1.0
+
-
|-
+
-
| enzyme 2 || 1.0
+
-
|-
+
-
| dH<sub>2</sub>O || ---
+
-
|-
+
-
| &nbsp; || 30 μL --> 37°C/ ~30 min.
+
-
|}
+
-
{| class="wikitable" width=400px
+
-
| Plasmid DNA || 15.0 μl*
+
-
|-
+
-
| Fermentas FastDigest enzyme 1 || 1.0 μl
+
-
|-
+
-
| Fermentas FastDigest enzyme 2 || 1.0 μl
+
-
|-
+
-
| 10x FastDigest buffer + green loading dye || 3.0 μl
+
-
|-
+
-
| dH<sub>2</sub>O || 10.0 μl
+
-
|-
+
-
| &nbsp; || 30.0 μl total
+
-
|-
+
-
| colspan="2" | *For low yield DNA, use up to 25 μL; decrease dH<sub>2</sub>O accordingly.<br>Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 37°C for 10 minutes.
+
-
|}
+
-
__NOTOC__
+

Revision as of 14:14, 4 April 2012

Search this Project

Customize your entry pages

Behzad's Notebook

Project Description

  • Project description coming soon
Personal tools