User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(4 intermediate revisions not shown.)
Line 13: Line 13:
|-  
|-  
|colspan="2"|
|colspan="2"|
-
==04/04/11==
 
-
<!-- Precede finished items with a checkmark &#x2713; -->
 
-
* &#x2713; Assemblies: BD111, BD112
 
-
----
 
-
'''Assemblies'''
 
-
# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
 
-
# KAH214: " + (2) KAH211/(X/P)/1321
 
-
# KAH215: " + (3) KAH212/(X/P)/1621
 
-
# KAH216: hPCD/(S/P)/186 &#x2713; + KAH210/(X/P)/2398
 
-
# KAH217: " + KAH211/(X/P)/1321
 
-
# KAH218: " + KAH212/(X/P)/1621
 
-
# KAH207/V0200: KAH207/(N/S)/1836 &#x2713; + V0200/(N/S dp)/4442
 
-
# KAH208/V0200: KAH208/(N/S)/1884 &#x2713; + "
 
-
# KAH209/V0200: KAH209/(N/S)/1932 &#x2713; + "
 
 +
==Behzad's Notebook==
-
> Digests (Fermentas FD)<br>
+
'''Project Description'''
-
 
+
* Project description coming soon
-
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
+
-
|-valign="top"
+
-
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
+
-
| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
+
-
|-
+
-
| DNA (plasmid) || up to 25 μL
+
-
|-
+
-
| 10x buffer || 3.0
+
-
|-
+
-
| enzyme 1 || 1.0
+
-
|-
+
-
| enzyme 2 || 1.0
+
-
|-
+
-
| dH<sub>2</sub>O || ---
+
-
|-
+
-
| &nbsp; || 30 μL --> 37°C/ ~30 min.
+
-
|}
+
-
 
+
-
__NOTOC__
+

Revision as of 14:14, 4 April 2012

Search this Project

Customize your entry pages

Behzad's Notebook

Project Description

  • Project description coming soon
Personal tools