User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
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| rowspan="9" |  
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| DNA (plasmid) || up to 20 μL
| DNA (plasmid) || up to 20 μL

Revision as of 21:50, 3 April 2012

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04/04/11

  • ✓ Assemblies: BD111, BD112, BD113

Assemblies

  1. BD111: KAH109/(X/P)/1123 + BBa_B0030/(S/P)/15
  2. BD112: BD111/(X/P)/1138 + BBa_I712074/(S/P)46
  3. BD113: BD111/(X/P)/1138 + BBa_I719005/(S/P)23


> Digests (Fermentas FD)

Reagent Volume  
DNA (plasmid) up to 20 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.


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