From OpenWetWare
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| | + | ==04/04/11== |
| | + | <!-- Precede finished items with a checkmark ✓ --> |
| | + | * ✓ Assemblies: BD111, BD112 |
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| | '''Assemblies''' | | '''Assemblies''' |
Revision as of 21:06, 3 April 2012
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Customize your entry pages 
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04/04/11
- ✓ Assemblies: BD111, BD112
Assemblies
- KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
- KAH214: " + (2) KAH211/(X/P)/1321
- KAH215: " + (3) KAH212/(X/P)/1621
- KAH216: hPCD/(S/P)/186 ✓ + KAH210/(X/P)/2398
- KAH217: " + KAH211/(X/P)/1321
- KAH218: " + KAH212/(X/P)/1621
- KAH207/V0200: KAH207/(N/S)/1836 ✓ + V0200/(N/S dp)/4442
- KAH208/V0200: KAH208/(N/S)/1884 ✓ + "
- KAH209/V0200: KAH209/(N/S)/1932 ✓ + "
> Digests (Fermentas FD)
| Reagent | Volume |
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30 μL/lane, 1% agarose -->
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| DNA (plasmid) | up to 25 μL
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| 10x buffer | 3.0
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| enzyme 1 | 1.0
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| enzyme 2 | 1.0
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| dH2O | ---
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| | 30 μL --> 37°C/ ~30 min.
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