User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 13: Line 13:
|-  
|-  
|colspan="2"|
|colspan="2"|
-
==Project Description/Abstract==
+
----
-
[[Image:engineering_pctfs.jpg|300px|thumb|right|]]
+
'''Assemblies'''
-
* Synthetic biology is an exciting new field that aims to create novel biological functions by artificially combining natural molecular parts. Rational design allows us to engineer the behaviors of protein-based drugs, in ways that we cannot accomplish with small molecule-based drugs. DNA assembly will be used to build a combinatorial library of over 200 synthetic transcription factors (Pc-Tfs) that will potentially activate therapeutic genes in cancer and stem cells. As time allows, a select group of Pc-TF protein will be purified and analyzed biochemically.
+
# KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
 +
# KAH214: " + (2) KAH211/(X/P)/1321
 +
# KAH215: " + (3) KAH212/(X/P)/1621
 +
# KAH216: hPCD/(S/P)/186 ✓ + KAH210/(X/P)/2398
 +
# KAH217: " + KAH211/(X/P)/1321
 +
# KAH218: " + KAH212/(X/P)/1621
 +
# KAH207/V0200: KAH207/(N/S)/1836 ✓ + V0200/(N/S dp)/4442
 +
# KAH208/V0200: KAH208/(N/S)/1884 ✓ + "
 +
# KAH209/V0200: KAH209/(N/S)/1932 ✓ + "
-
==Notes==
 
-
* Brady Laughlin's Spring 2012 project in the Haynes Lab at Arizona State University
 
-
* BME 394 Honors Research Project
 
 +
> Digests (Fermentas FD)<br>
 +
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
 +
|-valign="top"
 +
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
 +
| rowspan="9" | [[Image:KAH_071211_gel1.tif|200px|Cloning digests 7/12/11]]<br>30 μL/lane, 1% agarose -->
|-
|-
-
|colspan="2" style="background-color: #F2F2F2;"|
+
| DNA (plasmid) || up to 25 μL
-
{{LnNotebookRecentChanges2}}
+
|-
 +
| 10x buffer || 3.0
 +
|-
 +
| enzyme 1 || 1.0
 +
|-
 +
| enzyme 2 || 1.0
 +
|-
 +
| dH<sub>2</sub>O || ---
 +
|-
 +
| &nbsp; || 30 μL --> 37°C/ ~30 min.
|}
|}
__NOTOC__
__NOTOC__

Revision as of 21:02, 3 April 2012

Search this Project

Customize your entry pages

Assemblies

  1. KAH213: (4) KAH54/(S/P)/430 + (1) KAH210/(X/P)/2398
  2. KAH214: " + (2) KAH211/(X/P)/1321
  3. KAH215: " + (3) KAH212/(X/P)/1621
  4. KAH216: hPCD/(S/P)/186 ✓ + KAH210/(X/P)/2398
  5. KAH217: " + KAH211/(X/P)/1321
  6. KAH218: " + KAH212/(X/P)/1621
  7. KAH207/V0200: KAH207/(N/S)/1836 ✓ + V0200/(N/S dp)/4442
  8. KAH208/V0200: KAH208/(N/S)/1884 ✓ + "
  9. KAH209/V0200: KAH209/(N/S)/1932 ✓ + "


> Digests (Fermentas FD)

Reagent Volume   Cloning digests 7/12/11
30 μL/lane, 1% agarose -->
DNA (plasmid) up to 25 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.


Retrieved from "http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli"
Personal tools