User:Beatriz Gimenez De C./Notebook/572/2015/03/31: Difference between revisions

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** Fill up 29 culture tubes to 5 mL total with distilled water
** Fill up 29 culture tubes to 5 mL total with distilled water
** Placed in oven at 80ºC for 4h
** Placed in oven at 80ºC for 4h
* Prepare concentrated Tris/NaCl buffer (2.5 M Tris + 0.5 M NaCl)




Revision as of 11:44, 31 March 2015

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Tasks

  • Preparation of more AuNP Fibers
    • Prepare Lysozyme 20 μM stock solution
      • Measured 0.01439 g of lysozyme into a 50 mL volumetric flask and filled the rest with distilled water
    • Prepare 2.5×10-3 M HAuCl4 stock solution
      • added 0.04361 g of HAuCl4 into a 50 mL volumetric flask and filled the rest with distilled water
    • Transfer 0.5 mL 2.5×10-3 M HAuCl4 into 29 culture tubes
      • 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for a 45:1 ratio
    • Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes
      • 2.77777778×10-8(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio
    • Fill up 29 culture tubes to 5 mL total with distilled water
    • Placed in oven at 80ºC for 4h
  • Prepare concentrated Tris/NaCl buffer (2.5 M Tris + 0.5 M NaCl)




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