User:Beatriz Gimenez De C./Notebook/572/2015/01/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Task==
==Task==
* Set reactions between lysozyme-AuNp fiber and proteases tested, set 2 experiments per protease for a total of 10 reactions.  
* Set reactions between lysozyme-AuNp fiber and 4 different concentrations of pepsin, set 2 experiments per concentration for a total of 8 reactions.  
** System 1: AuNP/pepsin   
** '''System 1:''' Lysozyme-AuNP/57.8μM pepsin '''→''' 0.00530g of pepsin diluted in 1.325 mL of buffer + 2.65 mL of buffer to fibers (lasting 4 hours)
** System 2: AuNP/proteinase K
***** Lysozyme-AuNP/57.8μM pepsin  '''→''' 0.00398g of pepsin diluted in 1.00 mL of buffer + 2.00 mL of buffer to fibers (lasting 6 days)                   
** System 3: AuNP/thermolysin
** '''System 2:''' Lysozyme-AuNP/0.12μM pepsin  '''→''' 0.00391g of pepsin dilute in 1.00 mL of buffer, add 0.005 ml of solution + 5 mL of buffer to fibers (lasting 4 hours)
** System 4: AuNP/chymotrypsin
***** Lysozyme-AuNP/0.14μM pepsin  '''→''' 0.00470g of pepsin dilute in 1.00 mL of buffer, add 0.005 ml of solution + 5 mL of buffer to fibers (lasting 6 days)
** System 5: AuNP/trypsin  
** '''System 3:'''Lysozyme-AuNP/1.2μM pepsin  '''→''' 0.00419g of pepsin dilute in 1.00 mL of buffer, add 0.05 ml of solution + 4.95 mL of buffer to fibers (lasting 4 hours)
* 1 give a few hours and analyse that day
***** Lysozyme-AuNP/1.4μM pepsin  '''→''' 0.00465g of pepsin dilute in 1.00 mL of buffer, add 0.05 ml of solution + 4.95 mL of buffer to fibers (lasting 6 days)
* 1 let go for a week (analyse Tuesday)  
** '''System 4:''' Lysozyme-AuNP/12.5μM pepsin  '''→''' 0.00780g of pepsin dilute in 2.00 mL of buffer, add 0.5 ml of solution + 4.5 mL of buffer to fibers (lasting 4 hours)
***** Lysozyme-AuNP/12.6μM pepsin '''→''' 0.00589g of pepsin dilute in 1.50 mL of buffer, add 0.5 ml of solution + 4.5 mL of buffer to fibers (lasting 6 days)
* analysis for each system:
** 1 give a 4 hours and analyze that day
** 1 let go for a week (analyze Tuesday)  
* Color change reaction monitoring  
* Color change reaction monitoring  
** No color change was observed in the first 4 hours of observation.


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Latest revision as of 00:39, 27 September 2017

Project name Main project page
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Task

  • Set reactions between lysozyme-AuNp fiber and 4 different concentrations of pepsin, set 2 experiments per concentration for a total of 8 reactions.
    • System 1: Lysozyme-AuNP/57.8μM pepsin → 0.00530g of pepsin diluted in 1.325 mL of buffer + 2.65 mL of buffer to fibers (lasting 4 hours)
          • Lysozyme-AuNP/57.8μM pepsin → 0.00398g of pepsin diluted in 1.00 mL of buffer + 2.00 mL of buffer to fibers (lasting 6 days)
    • System 2: Lysozyme-AuNP/0.12μM pepsin → 0.00391g of pepsin dilute in 1.00 mL of buffer, add 0.005 ml of solution + 5 mL of buffer to fibers (lasting 4 hours)
          • Lysozyme-AuNP/0.14μM pepsin → 0.00470g of pepsin dilute in 1.00 mL of buffer, add 0.005 ml of solution + 5 mL of buffer to fibers (lasting 6 days)
    • System 3:Lysozyme-AuNP/1.2μM pepsin → 0.00419g of pepsin dilute in 1.00 mL of buffer, add 0.05 ml of solution + 4.95 mL of buffer to fibers (lasting 4 hours)
          • Lysozyme-AuNP/1.4μM pepsin → 0.00465g of pepsin dilute in 1.00 mL of buffer, add 0.05 ml of solution + 4.95 mL of buffer to fibers (lasting 6 days)
    • System 4: Lysozyme-AuNP/12.5μM pepsin → 0.00780g of pepsin dilute in 2.00 mL of buffer, add 0.5 ml of solution + 4.5 mL of buffer to fibers (lasting 4 hours)
          • Lysozyme-AuNP/12.6μM pepsin → 0.00589g of pepsin dilute in 1.50 mL of buffer, add 0.5 ml of solution + 4.5 mL of buffer to fibers (lasting 6 days)
  • analysis for each system:
    • 1 give a 4 hours and analyze that day
    • 1 let go for a week (analyze Tuesday)
  • Color change reaction monitoring
    • No color change was observed in the first 4 hours of observation.


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