User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions

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* Lysozyme-AuNP fibers preparation  
* Lysozyme-AuNP fibers preparation  
** Prepare Lysozyme stock (0.6 g/L lysozyme) makes the colloid precipitate out of solution. The fresh stock solution is to have a concentration of 0.6g/L, keeping the [Au]:[Lysozyme]=30:1.
** Prepare Lysozyme (0.6 g/L lysozyme) stock solution
* Transferred 0.5 mL 2.5×10<sup>-3</sup>M HAuCl<sub>4</sub> into 5 culture tubes
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution
* Max. protein concentration (double volume of protein as gold)=250μM/6=42μM
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into x culture tubes
* Since final volume of lysozyme stock solution needs to be 25 mL,
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for A 45:1 ratio
** (42×10<sup>-6</sup>mol/L)*(0.025L)*(14,307g/mol)=0.015g of lysozyme
** Transfer 0.661769570 mL of lysozyme stock solution into x culture tubes
** Made up to 50 mL mark with distilled water
*** 0.6(g/l)/14307(g/mol)= 4.1937510×10<sup>-5</sup>(mol/L)
* Added 1 mL from 42 μM lysozyme stock solution to make [Au:Lysozyme]=30
*** 2.77777778×10<sup>-8</sup>(mol)/4.1937510×10<sup>-5</sup>(mol/L)= 0.661769570 mL for a 45:1 ratio
* Filled up culture tubes to 5 mL total with water
** Fill up culture tubes to 5 mL total with water
* Placed in oven at 80ºC for 4h
** Placed in oven at 80ºC for 4h




Revision as of 11:41, 22 January 2015

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Tasks

  • Produce the lysozyme AuNP fibers.
  • Weigh the protease

Procedure

  • Lysozyme-AuNP fibers preparation
    • Prepare Lysozyme (0.6 g/L lysozyme) stock solution
    • Prepare 2.5×10-3 M HAuCl4 stock solution
    • Transfer 0.5 mL 2.5×10-3 M HAuCl4 into x culture tubes
      • 0.5×10-3(L)*2.5×10-3(mol/L)= 1.25 (mol) needed for A 45:1 ratio
    • Transfer 0.661769570 mL of lysozyme stock solution into x culture tubes
      • 0.6(g/l)/14307(g/mol)= 4.1937510×10-5(mol/L)
      • 2.77777778×10-8(mol)/4.1937510×10-5(mol/L)= 0.661769570 mL for a 45:1 ratio
    • Fill up culture tubes to 5 mL total with water
    • Placed in oven at 80ºC for 4h



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