User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions
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== | ==Tasks== | ||
* | * Produce the lysozyme AuNP fibers. | ||
* Weigh the protease | |||
==Procedure== | |||
* Lysozyme-AuNP fibers preparation | |||
** Prepare Lysozyme stock (0.6 g/L lysozyme) makes the colloid precipitate out of solution. The fresh stock solution is to have a concentration of 0.6g/L, keeping the [Au]:[Lysozyme]=30:1. | |||
* Transferred 0.5 mL 2.5×10<sup>-3</sup>M HAuCl<sub>4</sub> into 5 culture tubes | |||
* Max. protein concentration (double volume of protein as gold)=250μM/6=42μM | |||
* Since final volume of lysozyme stock solution needs to be 25 mL, | |||
** (42×10<sup>-6</sup>mol/L)*(0.025L)*(14,307g/mol)=0.015g of lysozyme | |||
** Made up to 50 mL mark with distilled water | |||
* Added 1 mL from 42 μM lysozyme stock solution to make [Au:Lysozyme]=30 | |||
* Filled up culture tubes to 5 mL total with water | |||
* Placed in oven at 80ºC for 4h | |||
Revision as of 11:10, 22 January 2015
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