User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Tasks== | ||
* | * Produce the lysozyme AuNP fibers (Made 50, 5 mL samples) | ||
* Weigh 4 mg of Pepsine protease into 8 tubes | |||
* Prepare buffer | |||
==Procedure== | |||
* Lysozyme-AuNP fibers preparation | |||
** Prepare Lysozyme 20 μM stock solution | |||
*** Measured 0.0261 g of lysozyme into a 100 mL volumetric flask and filled the rest with distilled water | |||
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | |||
*** added 0.08539 g of HAuCl4 into a 100 mL volumetric flask and filled the rest with distilled water | |||
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | |||
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for a 45:1 ratio | |||
** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | |||
*** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio | |||
** Fill up 50 culture tubes to 5 mL total with distilled water | |||
** Placed in oven at 80ºC for 4h | |||
*Weighed Protease pepsin into 8 tubes, for the next experiment. | |||
** 1. 0.00530 g | |||
** 2. 0.00398 g | |||
** 3. 0.00391 g | |||
** 4. 0.00470 g | |||
** 5. 0.00419 g | |||
** 6. 0.00465 g | |||
** 7. 0.00780 g | |||
** 8. 0.00584 g | |||
* Prepared 5 mM Tris/0.2 M NaCl buffer for reaction of the synthesized lysozyme-AuNP fibers with the proteases | |||
** 1000 mL of buffer was prepared, used 11.68960 g of NaCl + 0.78922 g of Tris in approx. 1000 mL of distilled water. | |||
** 0.5 N HCl added until pH=8.067. | |||
Latest revision as of 00:39, 27 September 2017
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Tasks
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