User:Beatriz Gimenez De C./Notebook/571/2014/10/29: Difference between revisions

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*Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
*Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
** Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
** Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
*** 50 mL of 0.6 g/L lysozyme solution were made using 0.0380 g of lysozyme.
*** 50 mL of 0.6 g/L lysozyme solution was made using 0.0380 g of lysozyme.
** Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.  
** Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.  


Revision as of 18:22, 31 October 2014

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Tasks

  • Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
    • Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
      • 50 mL of 0.6 g/L lysozyme solution was made using 0.0380 g of lysozyme.
    • Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.
  • Continue analysis of dialysis results from October 21 (started on October 28 with ISE data)
    • Bradford analysis
    • UV-Vis analysis
    • Fluorescence


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