User:Beatriz Gimenez De C./Notebook/571/2014/10/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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*Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
*Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
** Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
** Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
*** 50 mL of 0.6 g/L lysozyme solution were made using 0.0380 g of lysozyme.
*** 50 mL of 0.6 g/L lysozyme solution was made using 0.0380 g of lysozyme.
** Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.  
** Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.  


* Continue analysis of dialysis results from October 21 (started on October 28 with ISE data)
* Continue analysis of dialysis results from October 21 (started on October 28 with ISE data)
** KI calibration curve for ISE
[[Image:7.png]]
** Bradford analysis
** Bradford analysis
[[Image:5.png]]
** UV-Vis analysis
** UV-Vis analysis
** Fluorescence
[[Image:6.png]]


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Latest revision as of 00:29, 27 September 2017

Project name Main project page
Previous entry      Next entry

Tasks

  • Prepare dialysis of 0.6 g/L Lysozyme vs HCl using MWCO 20000
    • Prepare new 0.6 g/L lysozyme stock (need more in order to perform new dialysis)
      • 50 mL of 0.6 g/L lysozyme solution was made using 0.0380 g of lysozyme.
    • Prepare HCl stock solutions: 75 μM, 100 μM, 250 μM, 500 μM and 5 mM solutions through serial dilutions, with distilled water.
  • Continue analysis of dialysis results from October 21 (started on October 28 with ISE data)
    • KI calibration curve for ISE

    • Bradford analysis

    • UV-Vis analysis


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