User:Beatriz Gimenez De C./Notebook/571/2014/09/09: Difference between revisions

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*Transferred 0.5 mL 2.5×10mM HAuCl4, 167.5μL of the lysozime solution and 4.33 mL of water into 5 culture tube.
*Transferred 0.5 mL 2.5×10mM HAuCl4, 167.5μL of the lysozime solution and 4.33 mL of water into 5 culture tube.
*Covered test tubes with foil and placed all 5 test tubes in hot water beaker.
*Covered test tubes with foil and placed all 5 test tubes in hot water beaker.
*Recorded UV-Vis from 360 to 800 nm every 20 min over a period of 3h
*UV-Vis recorded from 800 nm to 360 nm until no more sample solution remained.


==Data==
==Data==


*Obtained spectra at time-points 0, 30, 50, 60, 80, 100, 120, 140, and 160  min


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Revision as of 16:37, 13 September 2014

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Tasks

  • spectra of [HAuCl4]Lysozime solution at 70°C
  • measure the fluorescence of tyrosine and histidine

Kinetics of Au:Lysozyme

  • Heated a 150 mL beaker of water to 70ºC
  • Made a 10mL solution of 250μM lysozime
  • Transferred 0.5 mL 2.5×10mM HAuCl4, 167.5μL of the lysozime solution and 4.33 mL of water into 5 culture tube.
  • Covered test tubes with foil and placed all 5 test tubes in hot water beaker.
  • UV-Vis recorded from 800 nm to 360 nm until no more sample solution remained.

Data

  • Obtained spectra at time-points 0, 30, 50, 60, 80, 100, 120, 140, and 160 min