User:Beatriz Gimenez De C./Notebook/571/2014/09/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*** 200 μmol/L × 131.17 g/mol × 0.1 L = 0.00262 g of isoleucine (calculation for all solutions: 0.00266 g of aspartic acid, 0.00310 g of histidine, 0.00362 g of tyrosine, 0.00178 g of alanine) | *** 200 μmol/L × 131.17 g/mol × 0.1 L = 0.00262 g of isoleucine (calculation for all solutions: 0.00266 g of aspartic acid, 0.00310 g of histidine, 0.00362 g of tyrosine, 0.00178 g of alanine) | ||
* Standardize titrant | * Standardize titrant | ||
** Potassium Hydrogen Phthalate (KHPhth) standard for NaOH. (0.501 g of KHPhth +17 mL of water + titrated with NaOH stock solution + 5 drops of Phenolphthalein/indicator) | ** Potassium Hydrogen Phthalate (KHPhth) standard for NaOH. (0.501 g of KHPhth +17 mL of water + titrated with NaOH stock solution + 5 drops of Phenolphthalein/indicator) | ||
** Titration repeated two times + yielded same result | ** Titration repeated two times + yielded same result | ||
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| 3||0.519||17.10 | | 3||0.519||17.10 | ||
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* Titrate Amino Acids | |||
** Isoleucine (Hydrophobic) | |||
*** 0.3896 g of Isoleucine + 10 mL of DI water + 10 mL of 21 mM HCl -> initial pH 3.351. | |||
*** 0.1485 M NaOH stock solution titrated 2 mL intervals until pH reached around 10 + pH recorded every 2 mL (titration curve). | |||
** Histidine It was suggested that amino acid solutions be made that would require 20 mL of NaOH to be titrated. | |||
*** 0.4608 g of Histidine + 10 mL of DI water + 10 mL of 21 mM HCl -> initial pH 2.538. | |||
*** 0.1485 M NaOH stock solution titrated 2 mL intervals until pH reached around 10 + pH recorded every 2 mL (titration curve). | |||
* UV/VIS Spectra | * UV/VIS Spectra | ||
* Fluorescence Spectra | * Fluorescence Spectra |
Latest revision as of 00:32, 27 September 2017
Project name | Main project page Next entry | ||||||||||||
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