User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/08/21: Difference between revisions
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Ok, I ran a gel today using the old extracts again, no reason to think they might be bad. I followed the protocol pretty closely. The only things that changed were - | |||
* | *I did 6μl of extract so that I could split in two. So I added 30μl of glyoxal reaction mix to each. This means that once I have added 50μl of formamide buffer that I can only add about half of the RNA to the gel. | ||
*My glyoxalated RNA samples sat on ice for about an hour because I needed to make and autoclave the BTPE. | |||
*I ran the gel for four hours | |||
*I only did 20 min washes in the denaturing buffer, neutralizing buffer, and 20xSSC. | |||
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Latest revision as of 18:17, 21 August 2008
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Ok, I ran a gel today using the old extracts again, no reason to think they might be bad. I followed the protocol pretty closely. The only things that changed were -
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