User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/24: Difference between revisions
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#Induce cells if necessary and lyse by the end of the day (or the next morning) | #Induce cells if necessary and lyse by the end of the day (or the next morning) | ||
==Primer design information== | |||
>wt | |||
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA | |||
>mutant | |||
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA | |||
wt16sProbe1 | |||
5'-GGT TGG ATC ACC TCC-3' 49 | |||
wt16sProbe2 | |||
5'-GTT GGA TCA CCT CCT TA-3' 48 | |||
o16sProbe1 | |||
5'-GTT GGA TCA TTG TGG-3' 44 | |||
o16sProbe2 | |||
5'-GTT GGA TCA TTG TGG TTA-3' 47 | |||
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Revision as of 09:35, 24 June 2008
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Primer design information>wt TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA >mutant TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA wt16sProbe1 5'-GGT TGG ATC ACC TCC-3' 49 wt16sProbe2 5'-GTT GGA TCA CCT CCT TA-3' 48 o16sProbe1 5'-GTT GGA TCA TTG TGG-3' 44 o16sProbe2 5'-GTT GGA TCA TTG TGG TTA-3' 47 |