User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/24: Difference between revisions
From OpenWetWare
Barry Canton (talk | contribs) No edit summary |
Barry Canton (talk | contribs) No edit summary |
||
| Line 7: | Line 7: | ||
| colspan="3"| | | colspan="3"| | ||
#Induce cells if necessary and lyse by the end of the day (or the next morning) | #Induce cells if necessary and lyse by the end of the day (or the next morning) | ||
==Primer design information== | |||
>wt | |||
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA | |||
>mutant | |||
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA | |||
wt16sProbe1 | |||
5'-GGT TGG ATC ACC TCC-3' 49 | |||
wt16sProbe2 | |||
5'-GTT GGA TCA CCT CCT TA-3' 48 | |||
o16sProbe1 | |||
5'-GTT GGA TCA TTG TGG-3' 44 | |||
o16sProbe2 | |||
5'-GTT GGA TCA TTG TGG TTA-3' 47 | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
Revision as of 09:35, 24 June 2008
| <html><img src="/images/c/c3/Resultset_previous.png" border="0"/></html>Previous entry |  T7 RNAP transcription of rRNA
|
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Primer design information>wt TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA >mutant TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTTA wt16sProbe1 5'-GGT TGG ATC ACC TCC-3' 49 wt16sProbe2 5'-GTT GGA TCA CCT CCT TA-3' 48 o16sProbe1 5'-GTT GGA TCA TTG TGG-3' 44 o16sProbe2 5'-GTT GGA TCA TTG TGG TTA-3' 47 | ||
